![]() ![]() Lee SJ, Liu J, Oh SH, Soker S, Atala A, Yoo JJ (2008) Development of a composite vascular scaffolding system that withstands physiological vascular conditions. Nanotechnol 11:681–691ĭinis TM, Elia R, Vidal G, Dermigny Q, Denoeud C, Kaplan DL, Egles C, Marin F (2015) 3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration. Sheikh FA, Ju HW, Lee JM, Moon BM, Park HJ, Lee OJ, Kim JH, Kim DK, Park CH (2015) 3D electrospun silk fibroin nanofibers for fabrication of artificial skin, Nanomed. Zhang Y, Venugopal JR, El-Turki A, Ramakrishna S, Su B, Lim CT (2008) Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Jiang T, Carbone EJ, Lo KWH, Laurencin CT (2015) Electrospinning of polymer nanofibers for tissue regeneration. Vasita R, Katti DS (2006) Nanofibers and their applications in tissue engineering. Zhang W, Tucker-Kellogg L, Narmada BC, Venkatraman L, Chang S, Lu Y, Tan N, White JK, Jia R, Bhowmick SS, Shen S, Dewey CF, Yu H (2010) Cell-delivery therapeutics for liver regeneration. Palakkan AA, Hay DC, Anil Kumar PR, Kumary TV, Ross JA (2013) Liver tissue engineering and cell sources: issues and challenges. Onodera K, Sakata H, Yonekawa M, Kawamura A (2006) Artificial liver support at present and in the future. These results indicated that the ordered 6 × 6 array scaffold has the potential as a suitable substratum for in vitro culture of hepatocytes. When HepG 2 cells were seeded on the arrayed scaffold, cells showed superior adhesion ability, better cell morphology and three-dimensional growth. Compared with the conventional scaffold, the pore size of the arrayed scaffold doubled and the hydrophilicity was improved. Specifically, a 6 × 6 array scaffold which was prepared by probe collector was orderly arrayed. Though increase in pore size decreases the tensile stress of scaffolds, it leads to enhance the proliferation and attachment of hepatocytes. We show that the average pore size of electrospun scaffold increases from ~ 7.6 to 13.2 μm, while the average fiber diameter decreases from ~ 2.0 to 1.5 μm when collected by probe array collectors. The pore size of scaffolds plays an important role in promoting the infiltration and proliferation of hepatocyte. doi: 10.1016/j.ydbio.2016.06.036.Nanofiber scaffolds are widely used as the platform for three-dimensional culture of hepatocytes in vitro. Reverse Engineering Liver Buds Through Self-Driven Condensation And Organization Towards Medical Application. Implementation of the Lancet Standing Commission on Liver Disease in the UK. The Lancet Commissions Addressing liver disease in the UK: a blueprint for attaining excellence in health care and reducing premature mortality from lifestyle issues of excess consumption of alcohol, obesity, and viral hepatitis. The burden of liver disease in Europe: A review of available epidemiological data. 2017 (2017).īlachier M, Leleu H, Peck-Radosavljevic M, Valla D-C, Roudot-Thoraval F. Blended protein:polymer scaffolds provide a viable, translatable niche for hepatocytes and offers a solution to current obstacles in disease modelling and liver tissue engineering. Each scaffold maintained hepatocyte growth, albumin production and influenced expression of key hepatic genes, with the decellularized ECM scaffolds exerting an influence which is not recapitulated by individual ECM components. Mechanical testing demonstrated significant increases in the Young's Modulus of the decellularized ECM scaffold providing significantly stiffer environments for hepatocytes. Immunohistochemistry confirmed retention of proteins in the scaffolds. The resulting scaffolds were validated using THLE-3 hepatocytes. We combined decellularized human liver tissue with electrospun polymers to produce a niche for hepatocytes and compared the human liver ECM to its individual components Collagen I, Laminin-521 and Fibronectin. Enhancing microenvironments using bioactive molecules allows researchers to create more appropriate niches for hepatocytes. Polymers and decellularized tissue scaffolds each provide some of the necessary biological cues for hepatocytes, however, neither alone has proved sufficient. One of the challenges for tissue engineers is the extracellular matrix (ECM) a finely controlled in vivo niche which supports hepatocytes. Tissue engineering of a transplantable liver could provide an alternative to donor livers for transplant, solving the problem of escalating donor shortages. ![]()
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